Next generation sequencing (NGS) technology has revolutionized genomic and genetic research. The pace of change in this area is rapid with three major new sequencing platforms having been released in 2011: Ion Torrent's PGM, Pacific Biosciences' RS and the Illumina MiSeq. Here we compare the results obtained with those platforms to the performance of the Illumina HiSeq, the current market leader. In order to compare these platforms, and get sufficient coverage depth to allow meaningful analysis, we have sequenced a set of 4 microbial genomes with mean GC content ranging from 19.3 to 67.7%. Together, these represent a comprehensive range of genome content. Here we report our analysis of that sequence data in terms of coverage distribution, bias, GC distribution, variant detection and accuracy. All three fast turnaround sequencers evaluated here were able to generate usable sequence. However there are key differences between the quality of that data and the applications it will support. The backmatter of the book contains a few articles concerning the merits of open access publishing.
Journalist Samela Harris is a working mum with a healthy sense of the ridiculous. While raising her two sons, she attracted droves of hungry lads to generous spreads cooked on her shoestring budget. Along the way she learnt how to create a dinner party from two-minute noodles, how to trick the boys into eating and enjoying prunes, and how to make a meal from scraps while accidentally locked in the kitchen.